Gastrointestinal Disease in Sows/Gilts/Boars
Required Forms:
OAHN Swine Small Scale Herd Postmortem Project-Survey
OAHN Swine Small Scale Herd Postmortem Project - Submission form
Gastrointestinal Disease in Sow/Gilt/Boar tissue sampling list
TISSUE SAMPLING LIST-collect the following samples from EACH pig
Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided. Formalin-fixed tissues: 1 sample jar per pig. |
Tissue | Fresh (include lesions) | In Formalin (include lesions) |
Tonsil | X | X |
Thyroid gland | X | |
Trachea | X | |
Thymus | X | |
Lung | X2 | X |
Heart | X | |
Liver | X | X |
Spleen | X | X |
Kidney | X | X |
Adrenal gland | X | |
Pancreas | X | |
Mesenteric lymph node | X | X |
Gastrohepatic lymph node | X | X |
Stomach | X | |
Duodenum | X | |
Jejunum | X | |
Ileum | X4 | X |
Spiral colon | X3 | X |
Feces | X | |
Diaphragm | X | |
Skeletal muscle | X | |
Brain (or head) | X half, sagitally sectioned (OR entire head, fresh) | X half sagitally sectioned (OR entire head, fresh) |
Gastrointestinal Disease in Sows /Gilts / Boars
Fresh tissues: Use a separate Whirl-Pak bag for each sample – label bags with the stickers provided (tissue name).
Formalin-fixed tissues: Use 1 sample jar per pig. For solid organs, samples should be approximately 1-2 cm2. For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface).
Test list (for AHL use - included on submission form):
Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung
Rotavirus A/B/C and Porcine coronavirus PCR rotapcr, pcorpcr: ileum
Bacterial culture bsetup, cults: ileum, colon (pool)
Lawsonia PCR lapcr: ileum
Brachyspira spp PCR brpcr and bspcr: colon
Parasitology – fecal flotation fflot: feces
Histopathology hist
Freeze and hold: tonsil / spleen, lung, kidney / liver, lymph nodes, ileum, colon, brain.