Lameness in Nursing Piglets

Required Forms:

OAHN Swine Small Scale Herd Postmortem Project-Survey

OAHN Swine Small Scale Herd Postmortem Project - Submission form

Lameness in Nursing Piglets tissue sampling list

TISSUE SAMPLING LIST-collect the following samples from EACH pig

Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided.

Formalin-fixed tissues: 1 sample jar per pig.

Tissue Fresh (include lesions) In Formalin (include lesions)

Tonsil

X

X

Thyroid gland

 

X

Trachea

 

X

Thymus

 

X

Lung

X 2

X

Heart

 

X

Liver

X

X

Spleen

X

X

Kidney

X

X

Adrenal gland

 

X

Pancreas

 

X

Mesenteric lymph node

X

X

Gastrohepatic lymph node

X

X

Stomach

 

X

Duodenum

 

X

Jejunum

 

X

Ileum

 

X

Spiral colon

 

X

Diaphragm

 

X

Skeletal muscle

 

X

Spinal cord or intact spinal column

X

X

Joints: 1 representative joint with lesions, per pig

synovium / joint capsule, swab, or aspirate X2

synovium / joint capsule

Brain (OR submit head intact) X half, sagitally sectioned X half, sagitally sectioned

Lameness in Nursing Piglets (1-3 animals)

Fresh tissues: Use a separate Whirl-Pak bag for each sample –label bags with the stickers provided (tissue name), and add a pig designation (A, B, C – if >1 pig).

Formalin-fixed tissues: Use 1 sample jar per pig, and label jar with the appropriate pig designation (A, B, C). For solid organs, samples should be approximately 1-2  cm2.  For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface). 

Test list (for AHL use – included on submission form) :

Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung (pool)

Bacterial culture bsetup, cults: joint swab, joint capsule, or aspirate (culture individually)

Mycoplasmology: M.hyorhinis PCR hrhpcr: joint swab, joint capsule, or aspirate (pool)

Histopathology hist

Freeze and hold: tonsil, spleen, lung, kidney, liver, lymph nodes, brain, synovium /joint capsule or swab / aspirate.