Lameness in Nursing Piglets
Required Forms:
OAHN Swine Small Scale Herd Postmortem Project-Survey
OAHN Swine Small Scale Herd Postmortem Project - Submission form
Lameness in Nursing Piglets tissue sampling list
TISSUE SAMPLING LIST-collect the following samples from EACH pig
Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided. Formalin-fixed tissues: 1 sample jar per pig. |
Tissue | Fresh (include lesions) | In Formalin (include lesions) |
Tonsil |
X |
X |
Thyroid gland |
|
X |
Trachea |
|
X |
Thymus |
|
X |
Lung |
X 2 |
X |
Heart |
|
X |
Liver |
X |
X |
Spleen |
X |
X |
Kidney |
X |
X |
Adrenal gland |
|
X |
Pancreas |
|
X |
Mesenteric lymph node |
X |
X |
Gastrohepatic lymph node |
X |
X |
Stomach |
|
X |
Duodenum |
|
X |
Jejunum |
|
X |
Ileum |
|
X |
Spiral colon |
|
X |
Diaphragm |
|
X |
Skeletal muscle |
|
X |
Spinal cord or intact spinal column |
X |
X |
Joints: 1 representative joint with lesions, per pig |
synovium / joint capsule, swab, or aspirate X2 |
synovium / joint capsule |
Brain (OR submit head intact) | X half, sagitally sectioned | X half, sagitally sectioned |
Lameness in Nursing Piglets (1-3 animals)
Fresh tissues: Use a separate Whirl-Pak bag for each sample –label bags with the stickers provided (tissue name), and add a pig designation (A, B, C – if >1 pig).
Formalin-fixed tissues: Use 1 sample jar per pig, and label jar with the appropriate pig designation (A, B, C). For solid organs, samples should be approximately 1-2 cm2. For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface).
Test list (for AHL use – included on submission form) :
Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung (pool)
Bacterial culture bsetup, cults: joint swab, joint capsule, or aspirate (culture individually)
Mycoplasmology: M.hyorhinis PCR hrhpcr: joint swab, joint capsule, or aspirate (pool)
Histopathology hist
Freeze and hold: tonsil, spleen, lung, kidney, liver, lymph nodes, brain, synovium /joint capsule or swab / aspirate.