Neurologic Disease in Nursing Piglets

Required Forms:

OAHN Swine Small Scale Herd Postmortem Project-Survey

OAHN Swine Small Scale Herd Postmortem Project - Submission form

Neurologic Disease in Nursing Piglets tissue sampling list

TISSUE SAMPLING LIST-collect the following samples from EACH pig

Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided.

Formalin-fixed tissues: 1 sample jar per pig.

Tissue Fresh (include lesions) In Formalin (include lesions)
Tonsil X X
Thyroid gland   X
Trachea   X
Thymus   X
Lung X2 X
Heart   X
Liver X X
Spleen X X
Kidney X X
Adrenal gland   X
Pancreas   X
Mesenteric lymph node X X
Gastrohepatic lymph node X X
Stomach   X
Duodenum   X
Jejunum   X
Ileum   X
Spiral colon    X
Diaphragm   X
Skeletal muscle   X
Brain (or submit head intact) X half, sagitally sectioned AND meningeal swab X half, sagitally sectioned
Spinal cord (OR vertebral column removed en bloc) X X

Neurologic Disease in Nursing Piglets (1-3 animals)

Fresh tissues: Use a separate Whirl-Pak bag for each sample –label bags with the stickers provided (tissue name), and add a pig designation (A, B, C – if >1 pig).

Formalin-fixed tissues: Use 1 sample jar per pig and label with the pig designation (A, B, C). For solid organs, samples should be approximately 1-2  cm2.  For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface). 

Test list (for AHL use - included on submission form):

Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung (pool)

                   PHEV PCR hevrt: brainstem (pool)

Bacterial culture bsetup, cults: meningeal swab(s) (cultur individually)

Histopathology hist

Freeze and hold: tonsil / spleen, lung, kidney / liver, lymph nodes, brain, spinal cord.