Neurologic Disease in Sows /Gilts / Boars

Required Forms:

OAHN Swine Small Scale Herd Postmortem Project-Survey

OAHN Swine Small Scale Herd Postmortem Project - Submission form

Neurologic Disease in Sow/Gilt/Boar tissue sampling list

TISSUE SAMPLING LIST-collect the following samples from EACH pig

Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided.

Formalin-fixed tissues: 1 sample jar per pig.

Tissue Fresh (including lesions)   In formalin (including lesions) 
Tonsil X X
Thyroid gland   X
Trachea   X
Thymus   X
Lung X2 X
Heart   X
Liver X X
Spleen X X
Kidney X X
Adrenal gland   X
Pancreas   X
Mesenteric lymph node X X
Gastrohepatic lymph node X X
Stomach   X
Duodenum   X
Jejunum   X
Ileum   X
Spiral colon   X
Diaphragm and skeletal muscle   X
Brain (OR submit head intact) X half, sagitally sectioned AND meningeal swab X half, sagitally sectioned
Spinal cord (or vertebral column removed en bloc) X X

Neurologic Disease in Sows /Gilts / Boars (1 animal per case)

Fresh tissues: Use a separate Whirl-Pak bag for each sample –label bags with the stickers provided (tissue name).

Formalin-fixed tissues: Use 1 sample jar per pig. For solid organs, samples should be approximately 1-2  cm2.  For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface). 

Test list (for AHL use – included on submission form) :

Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung

Bacterial culture bsetup, cults: meningeal swab

Histopathology hist

Freeze and hold: tonsil / spleen, lung, kidney / liver, lymph nodes, brain, spinal cord.