Neurologic Disease in Grower-Finisher Pigs
Required Forms:
OAHN Swine Small Scale Herd Postmortem Project-Survey
OAHN Swine Small Scale Herd Postmortem Project - Submission form
Neurologic Disease in Grower-Finisher Pigs tissue sampling list
TISSUE SAMPLING LIST-collect the following samples from EACH pig
Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided. Formalin-fixed tissues: 1 sample jar per pig. |
Tissue | Fresh (include lesions) | In Formalin (include lesions |
Tonsil |
X |
X |
Thyroid gland |
|
X |
Trachea |
|
X |
Thymus |
|
X |
Lung |
X 2 |
X |
Heart |
|
X |
Liver |
X |
X |
Spleen |
X |
X |
Kidney |
X |
X |
Adrenal gland |
|
X |
Pancreas |
|
X |
Mesenteric lymph node |
X |
X |
Gastrohepatic lymph node |
X |
X |
Stomach |
|
X |
Duodenum |
|
X |
Jejunum |
|
X |
Ileum |
|
X |
Spiral colon |
|
X |
Diaphragm |
|
X |
Skeletal muscle |
|
X |
Brain (OR submit head intact) |
X half, sagitally sectioned AND meningeal swab |
X half, sagitally sectioned |
Spinal cord (OR vertebral column removed en bloc) |
X |
X |
Neurologic Disease in Grower-Finisher Pigs (>20 kg)
If <40 kg each, may include up to 3 pigs per case
Fresh tissues: Use a separate Whirl-Pak bag for each sample – label bags with the stickers provided (tissue name), and add a pig designation (A, B, C – if >1 pig).
Formalin-fixed tissues: Use 1 sample jar per pig and label with appropriate pig designation (A, B, C). For solid organs, samples should be approximately 1-2 cm2. For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface).
Test list (for AHL use - included on submission form):
Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung (pool)
Bacterial culture bsetup, cults: meningeal swab(s) (culture individually)
Histopathology hist
Freeze and hold: tonsil / spleen, lung, kidney / liver, lymph nodes, brain, spinal cord.