Lameness in Grower-Finisher Pigs
Required Forms:
OAHN Swine Small Scale Herd Postmortem Project-Survey
OAHN Swine Small Scale Herd Postmortem Project - Submission form
Lameness in Grower-Finisher Pigs tissue sampling list
TISSUE SAMPLING LIST-collect the following samples from EACH pig
|
Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided. Formalin-fixed tissues: 1 sample jar per pig. |
| Tissue | Fresh (include lesions) | In Formalin (include lesions) |
|
Tonsil |
X |
X |
|
Thyroid gland |
|
X |
|
Trachea |
|
X |
|
Thymus |
|
X |
|
Lung |
X 2 |
X |
|
Heart |
|
X |
|
Liver |
X |
X |
|
Spleen |
X |
X |
|
Kidney |
X |
X |
|
Adrenal gland |
|
X |
|
Pancreas |
|
X |
|
Mesenteric lymph node |
X |
X |
|
Gastrohepatic lymph node |
X |
X |
|
Stomach |
|
X |
|
Duodenum |
|
X |
|
Jejunum and Ileum |
|
X |
|
Spiral colon |
|
X |
|
Diaphragm and skeletal muscle |
|
X |
|
Joints: 1 representative joint with lesions, per pig |
synovium / joint capsule, swab, or aspirate X2 |
synovium / joint capsule |
|
Brain (OR submit head intact) |
X half sagitally sectioned AND meningeal swab |
X half sagitally sectioned |
|
Spinal cord (OR vertebral column removed en bloc) |
X |
X |
Lameness in Grower-Finisher Pigs (>20 kg)
If <40 kg each, may include up to 3 pigs per case
Fresh tissues: Use a separate Whirl-Pak bag for each sample – use stickers and marker to label bags with the tissue name and pig designation (pig A, B, or C).
Formalin-fixed tissues: Use 1 sample jar per pig and label with pig designation (pig A, B, or C). For solid organs, samples should be approximately 1-2 cm2. For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface).
Test list (for AHL use – included on submission form) :
Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung (pool)
Bacterial culture bsetup, cults: joint swab(s), joint capsule, or aspirate (pool by pig)
Mycoplasmology: M.hyosynoviae and M.hyorhinis PCR hsypcr, hrhpcr: joint swab(s), joint capsule, or aspirate (pool by pig)
Histopathology hist
Freeze and hold: tonsil / spleen, lung, kidney / liver, lymph nodes, brain, spinal cord.