Respiratory Disease in Nursery (Weaner) Pigs (<20 kg,

Required Forms:

OAHN Swine Small Scale Herd Postmortem Project-Survey

OAHN Swine Small Scale Herd Postmortem Project - Submission form

Respiratory Disease in Nursery (weaner) Pigs tissue sampling list

TISSUE SAMPLING LIST-collect the following samples from EACH pig

Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided.

Formalin-fixed tissues: 1 sample jar per pig.

Tissue  Fresh (include lesions) In Formalin (include lesions)

Tonsil

X

X

Thyroid gland

 

X

Trachea

 

X

Thymus

 

X

Lung

X 4

X

Heart

 

X

Liver

X

X

Spleen

X

X

Kidney

X

X

Adrenal gland

 

X

Pancreas

 

X

Mesenteric lymph node

X

X

Gastrohepatic lymph node

X

X

Stomach

 

X

Duodenum

 

X

Jejunum

 

X

Ileum

 

X

Spiral colon

 

X

Diaphragm

 

X

Skeletal muscle

 

X

Brain

(or head)

X half, sagitally sectioned (OR entire head, fresh)

X half, sagitally sectioned

(OR entire head, fresh)

Respiratory Disease in Nursery (Weaner) Pigs (<20 kg, 1-3 animals)

Fresh tissues: Use a separate Whirl-Pak bag for each sample – use provided stickers and marker to label the bag with the tissue name and pig designation (pig A, B, or C).

Formalin-fixed tissues: Use 1 sample jar per pig and label with pig designation (pig A, B, or C). For solid organs, samples should be approximately 1-2  cm2.  For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface). 

Test list (for AHL use - included on submission form):

Virology: PRRSV PCR prtqn,  influenza A virus PCR inflpcr: lung (pool)

Mycoplasmology: M.hyopneumoniae PCR mhpcr: lung (pool)

Bacterial culture bsetup, cults: lung (pool).

Histopathology hist

Freeze and hold: tonsil /spleen, lung, kidney /liver, lymph nodes, brain.