Sudden Death in Nursing Piglets
Required Forms:
OAHN Swine Small Scale Herd Postmortem Project-Survey
OAHN Swine Small Scale Herd Postmortem Project - Submission form
Sudden Death in Nursing Piglets tissue sampling list
TISSUE SAMPLING LIST-collect the following samples from EACH pig
Fresh tissues: 1 sample per Whirl-Pak bag, per pig – label with stickers provided. Formalin-fixed tissues: 1 sample jar per pig. |
Tissue | Fresh (include lesions) | In Formalin (include lesions) |
Tonsil | X | X |
Thyroid gland | X | |
Trachea | X | |
Thymus | X | |
Lung | X3 | X |
Heart | X | |
Liver | X | X |
Spleen | X2 | X |
Kidney | X | X |
Adrenal gland | X | |
Pancreas | X | |
Mesenteric lymph node | X | X |
Gastrohepatic lymph node | X | X |
Stomach | X | |
Duodenum | X | |
Jejunum | X | |
Ileum | X | |
Spiral colon | X | |
Diaphragm | X | |
Skeletal muscle | X | |
Brain (or head) | X half, sagitally sectioned (OR entire head-fresh) | X half, sagitally sectioned (OR entire head-fresh) |
Sudden Death in Nursing Piglets
Fresh tissues: Use a separate Whirl-Pak bag for each sample – use provided stickers and marker to label the bag with the tissue name and pig designation (pig A, B, or C).
Formalin-fixed tissues: Use 1 sample jar per pig and label with pig designation (pig A, B, or C). For solid organs, samples should be approximately 1-2 cm2. For intestine, take approximately 3 cm long segments and partially open longitudinally with scissors (to allow formalin to contact the mucosal surface).
Test list (for AHL use):
Virology: PRRSV PCR prtqn, influenza A virus PCR inflpcr: lung (pooled)
Bacterial culture bsetup, cults: lung, spleen (pooled by tissue)
Histopathology hist
Freeze and hold: tonsil /spleen, lung, kidney/ liver, lymph nodes, brain.